A new customer application report available from Genevac discusses an improved method for the preparation of samples for the mass spectroscopic detection of synthetic insulin in blood serum.

Recent developments in applying mass spectrometry to proteomics means that it is becoming feasible for doping laboratories to routinely detect and confirm the abuse of peptide hormones by sportsmen and women. Using mass spectrometry to detect and confirm the abuse of peptide hormones is preferred to the use of immunoassay techniques as the methodology is able to positively differentiate between endogenous insulin and its synthetic analogues. To use LC-MS techniques to detect synthetic insulin however requires that samples are extracted and concentrated prior to analysis. This traditionally time consuming procedure requires at least one evaporation of a solution with high water content.

Using a miVac Duo sample professional concentrator the authors of the report were able to significantly improve the sample preparation method for synthetic insulin’s in serum. Using the miVac Duo - sample preparation times were reduced from several days to just 2 hours and better and more consistent recoveries were obtained than using the traditional nitrogen evaporation method.

Affordably priced miVac sets the standard for careful and speedy sample concentration and drying from a variety of sample formats including tubes, microwell plates and vials. Compact in design - miVac concentrators feature built-in special methods for working with alcohols, water and water mixtures to improve performance and optimise concentration times.
1-845-267-2211 / salesinfo@genevac.com (North America).